Serum IgE in Guinea Pigs Died of Anaphylactic Shock and Its Forensic Signifi- cance When the Guinea Pig Cannot be Timely Refrigerated / 法医学杂志

OBJECTIVE@#To investigate the serum IgE with various postmortem intervals (PMI) in guinea pigs due to sudden death from anaphylactic shock and to explore the effect of refrigeration of corpse on serum IgE level and its application value in forensic medicine.@*METHODS@#The animal death models of anaphylactic shock were established. The corpses were preserved at room temperature (20 °C ) for 6 h and then refrigerated at 4 °C. The serum was sampled at 6, 12, 24, 36 and 48 hours after death. The IgE level of serum was detected with ELISA. The control group was also established.@*RESULTS@#The serum IgE level had significant. difference between the experimental group and the control group (P < 0.05). There was no significant difference among the experimental groups at 6, 12, 24, 36 and 48 hours post- mortem (P > 0.05).@*CONCLUSION@#If the corpses were placed in 4 °C conditions 6 hours after anaphylactic death, the serum IgE still shows a good marker within 48 h for forensic investigation.

Changes of Serum IgE and Tryptase in Anaphylactic Shock Rats / 法医学杂志

OJECTIVE@#To explore the changes of serum IgE and tryptase caused by anaphylactic shock rats and discuss the relation to PMI and preservative environment of corpse and specimen.@*METHODS@#Rats were used for establishing anaphylactic shock models and randomly divided into room temperature group, refrigeration group, frozen group, manual hemolysis group, specimen preservation group. And the control group was also established. The blood samples were collected after rats were sacrificed. The degree of hemolysis was graded according to the color of the upper layer of the serum. The mass concentration of IgE and tryptase in each group was detected by ELISA.@*RESULTS@#The levels of serum IgE and tryptase in anaphylactic shock dead rats were higher than that of the control group. Room temperature and frozen made obviously differences on the levels of serum IgE and tryptase with various PMI. The levels of serum IgE and tryptase in refrigeration group showed relatively stable. The levels of serum tryptase and IgE were elevated with differently increasing hemolysis. The levels of serum IgE and tryptase showed no obvious changes during the specimen kept under different temperature conditions for 25 days.@*CONCLUSION@#Serum IgE and tryptase obviously increased in anaphylactic shock rats. However, the levels were influenced by PMI and environmental temperature, especially under the conditions of room temperature and frozen.

Diseño y validación de un instrumento para el registro de reacciones adversas a la donación de sangre y sus componentes / Design and validation of a program for the registration of adverse reactions to blood donation and its components", "_i": "

Introducción: La donación de sangre y sus componentes es un procedimiento en el que intervienen elementos internos y externos, los que al interactuar pueden convertirse en factores determinantes para desencadenar una reacción adversa durante la donación; ésta se entiende como un evento inesperado que pone en riesgo la salud del donante. Objetivo: Diseñar y validar un instrumento para registrar las reacciones adversas durante la donación de sangre y sus componentes que apoye la práctica de la enfermería de manera sistemática y ordenada. Material y métodosEstudio no experimental, transversal y prospectivo, en el que se diseñó un instrumento para el registro de reacción adversa durante la donación. Se validó por opinión de expertos de diferentes bancos de sangre de instituciones públicas en el período del 01 de enero al 31 de diciembre de 2011. Para determinar la consistencia interna, se utilizó la prueba de alfa de Cronbach con datos de 60 donantes que presentaron reacción adversa durante la donación durante o después del proceso de extracción de sangre. Resultados: El instrumento diseñado se integra de acuerdo con lo revisado en la literatura y con las observaciones y sugerencias aportadas por los expertos. Para procesar los datos se utilizó el programa SPSS versión 2.0; para calcular la confiabilidad del instrumento se determinó la consistencia del alfa de Cronbach, la cual arrojó un coeficiente de confiabilidad de α = 0.915 con 134 elementos analizados. Conclusiones: Se logra la validación y se cumple el objetivo de contar con un instrumento que permite el registro de reacción adversa durante la donación, apoyando el actuar de enfermería de manera ordenada y sistemática.

Introduction: The donation of blood and blood components is a procedure that involves internal an external elements which may become by interacting, determinans to trigger an adverse reaction during the donation; this is understood to be an unexpected event that threatens donor health. Objective: To design and validate and instrument to record adverse reactions to blood and its components donation in a systematic an orderly way to support nursing practice. Material and methods: Non-experimental, cross-sectional and prospective study in which and instrument was designed to record adverse reactions to donation. The instrument was validate by experts from different public blood banks in the period from January 1 to December 31, 2011. To determine the internal consistency, we used Cronbach’s alpha test with data from 60 donors who present adverse reaction during or after blood extraction procedure. Results: The designed instrument was integrated according to the reviewed literature and the comments and suggestions made be experts. To process the data, we used SPSS software version 2.0; to calculate the reliability of the instrument, we determined the consistency of Cronbach’s alpha, which yielded reliability coefficient of α = 0.915 with 134 elements analyzed. Conclusions: The validation is achieved and meets the objective of having an instrument that enables the recording of adverse reaction to blood and its components donation, thus supporting nursing performance in a systematic and orderly way.

Murine Model of Buckwheat Allergy by Intragastric Sensitization with Fresh Buckwheat Flour Extract

Food allergies affect about 4% of the Korean population, and buckwheat allergy is one of the most severe food allergies in Korea. The purpose of the present study was to develop a murine model of IgE-mediated buckwheat hypersensitivity induced by intragastric sensitization. Young female C3H/HeJ mice were sensitized and challenged intragastricly with fresh buckwheat flour (1, 5, 25 mg/dose of proteins) mixed in cholera toxin, followed by intragastric challenge. Anaphylactic reactions, antigen-specific antibodies, splenocytes proliferation assays and cytokine productions were evaluated. Oral buckwheat challenges of sensitized mice provoked anaphylactic reactions such as severe scratch, perioral/periorbital swellings, or decreased activity. Reactions were associated with elevated levels of buckwheatspecific IgE antibodies. Splenocytes from buckwheat allergic mice exhibited significantly greater proliferative responses to buckwheat than non-allergic mice. Buckwheat-stimulated IL-4, IL-5, and INF-gamma productions were associated with elevated levels of buckwheat-specific IgE in sensitized mice. In this model, 1 mg and 5 mg dose of sensitization produced almost the same degree of Th2-directed immune response, however, a 25 mg dose showed blunted antibody responses. In conclusion, we developed IgE-mediated buckwheat allergy by intragastric sensitization and challenge, and this model could provide a good tool for future studies.

Anaphylactoid transfusion reaction with anti-IgA antibodies in an IgA deficient patient: a case report.

A case of anaphylactoid transfusion reaction associated with anti-IgA antibodies is reported. The patient had low levels of serum IgA. She developed serious reaction characterised by erythematous rash, pruritus, cyanosis and dyspnoea after transfusion of otherwise compatible blood. Review of literature reveals that anaphylactoid transfusion reactions are very rare although frequency of anti-IgA in general population is quite high. The IgA deficient donors (aIgA) form a unique resource of blood components for the clinical management of patients with anaphylactoid reactions caused by anti-IgA.